lab1notes 1 - Microscopy; Cellularvs.Colonial Morphology

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Microscopy;  Cellular vs. Colonial  Morphology
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Brightfield Microscopy Light passes directly through specimen into the  objective lens, and up to the ocular lens to produce  an image Background appears white and any color of the  specimen or stain used can be distinguished from the  background
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Phase Contrast Microscopy Enhances contrasts of transparent and colorless objects by  influencing the optical path of light Able to show denser components in a cell or bacteria  without staining Light rays passing through high density area is refracted  differently from less dense areas = shifted out of phase Special phase contrast condenser collects in phase light to  produce light areas in image, out of phase refracted light  doesn’t escapes and dark area are produced  
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Microscopy Brightfield Phase  Contrast
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Microscope Parts Lenses Ocular: 10X Objectives: 4X, 10X, 40X, 100X
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This note was uploaded on 09/06/2011 for the course MIBO 3510L taught by Professor Dustman during the Spring '08 term at University of Georgia Athens.

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lab1notes 1 - Microscopy; Cellularvs.Colonial Morphology

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