Lab2notes3

Lab2notes3 - SmearPreparation SmearPreparation...

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Simple Staining Simple Staining
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Smear Preparation Smear Preparation Don’t forget  to flame the  loop before  you remove  some of the  culture! Don’t forget  to flame the  loop after  you’ve  made the  smear!
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How Much Is Enough? How Much Is Enough? Density of the smear is important to achieve  accurate staining results Aim for a skim milk like appearance in the  wet smear Solid medias: 1 loopful is more than enough Liquid medias: 3-4 loopfuls or more may be  needed Not sure if the density is good?    Err on the side of too thin, and let the smear dry.   You can always add a little bit more if necessary.
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Smear Density Comparison Smear Density Comparison
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Heat Fixation Heat Fixation Heat fixation ensures: Cells will remain adhered to the glass slide Cells are killed, preventing release of degrading 
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This note was uploaded on 09/06/2011 for the course MIBO 3510L taught by Professor Dustman during the Spring '08 term at UGA.

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Lab2notes3 - SmearPreparation SmearPreparation...

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