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Lab2notes 1 - Endospore&AcidFast Endospore&AcidFast...

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Endospore & Acid Fast  Endospore & Acid Fast  Staining Procedures Staining Procedures
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Endospore Staining Endospore Staining Endospores found in  Bacillus   (aerobic) and  Clostridium  (anaerobic) Dormant stage and highly resistant to  dehydration, chemicals, radiation Position inside vegetative cell is  indicative of species- central, terminal,  sub-terminal Contains dipicolinic acid (DPA) which  aids in endospore’s resistance
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Endospore Stain Procedure Endospore Stain Procedure Change in procedure: Do  NOT  use the paper towel. Instead, prevent the stain from  drying onto the slide by adding  additional stain drops during  primary staining .
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Endospore Stain Procedure Endospore Stain Procedure 1.   A bacterial smear is made and fixed. 2.   The smear is flooded with malachite green over a  steam bath  for 3 to 5 minutes  -primary stain-  then removed and cooled.
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