Lab2notes 2 - GramStain GramStain GramStainProcedure...

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Gram Stain Gram Stain
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Gram Stain Procedure Gram Stain Procedure A bacterial smear is made and fixed. The smear is flooded with crystal violet  -primary stain-  then  rinsed with water. The smear is flooded with Gram’s (or Lugol’s) iodine  -mordant-  then rinsed with water. The smear is rinsed with 95% ethanol briefly  -decolorizer-  then rinsed with water. The smear is flooded with safranin  -counterstain-  then  rinsed with water. The stained smear is blotted dry and viewed under oil  immersion.
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Gram Stain Chemistry Gram Stain Chemistry Finished Step Gram + Gram - Fixed smear Colorless cells Colorless cells Crystal violet Purple cells Purple cells Gram’s iodine Purple cells Purple cells Ethanol Purple cells Colorless cells Safranin Purple cells Red/pink cells
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Gram Stain Chemistry Gram Stain Chemistry Gram’s iodine complexes with crystal  violet to form an insoluble precipitate 
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This note was uploaded on 09/06/2011 for the course MIBO 3510L taught by Professor Dustman during the Spring '08 term at University of Georgia Athens.

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Lab2notes 2 - GramStain GramStain GramStainProcedure...

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