SDS-page - Biochemistry Lab SDS POLYACRYLAMIDE GEL...

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Biochemistry Lab November 26, 2005 SDS POLYACRYLAMIDE GEL ELECTROPHORESIS Objectives: SDS Polyacrylamide Gel Electrophoresis is a useful tool when determining the sizes of proteins. Differing sized proteins will move along a straight line through a polyacrylamide matrix. Since the proteins are different sizes, they will move at different rates through the polyacrylamide gel. With a standard run next to our sample proteins we can make a plot of the logarithm of the fragment size on the y- axis and the distance traveled in millimeters or centimeters on the x-axis. This will allow us to measure the distance traveled of our target protein and follow the straight line up and over to the y-axis to determine our protein size. The SDS in SDS-page stands for the type of detergent used, sodium dodecyl sulphate. SDS coats the protein in a negative charge to disseminate the charge-charge interactions present in proteins. During this experiment we learned how to purify the protein and how to visualize this purification.
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Data: A B C D E A=Standard B=Crude C= P1 D=P2 E=Standard
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SDS-page - Biochemistry Lab SDS POLYACRYLAMIDE GEL...

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