Hill reaction report - Using the Hill Reaction in Isolated...

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1-10-05 Using the Hill Reaction in Isolated Chloroplasts to Explore the Rate of Reactions at Varying Conditions Objectives : In this experiment we varied reaction mixtures and measured the absorbance in the spectrophotometer. The variables in part one of our experiment was to test possible inhibitors of the Hill reaction. We tested the inhibitors by using four different tubes, one with ammonia, one with DCMU, and the other two as controls. In part two of our experiment, we measured the affect of distance from a light source on the rate of the Hill reaction. The goal of these experiments was to explore a few of the variables that affect the Hill reaction and learn how the photo system works. Table 1: Experiment 1 Time Tube 0 1 2 3 4 5 1 0.558 2 0.815 0.554 0.404 0.289 0.163 0.03 3 0.559 0.191 0.008 0.005 0.002 0.002 4 0.603 0.588 0.581 0.574 0.57 0.561 Table 2 Experiment 2 Tube 0 1 2 3 4 5 Intensity 1 0.629 0.307 0.183 0.108 0.108 0.101 0.01 2 0.597 0.314 0.139 0.09 0.064 0.032 0.0025 3 0.409 0.217 0.1 0.016 0.01 0.008 0.0011 1
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1-10-05 Experiment 1: Graph 1 Change in Absorbance Over Time 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 0 1 2 3 4 5 Time (min) Change in Absorbance Tube 2 Tube 3 Tube 4 Experiment 2: Graph 2 Change in Absorbance vs. Time 0 0.1 0.2 0.3 0.4 0.5 0.6 0 1 2 3 4 5 Time (min.) Change in Absorbance 10 cm 20 cm 30 cm 2
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