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1-10-05Using the Hill Reaction in Isolated Chloroplasts to Explore the Rate of Reactions at Varying ConditionsObjectives:In this experiment we varied reaction mixtures and measured the absorbance in the spectrophotometer. The variables in part one of our experiment was to test possible inhibitors of the Hill reaction. We tested the inhibitors by using four different tubes, one with ammonia, one with DCMU, and the other two as controls. In part two of our experiment, we measured the affect of distance from a light source on the rate of the Hill reaction. The goal of these experiments was to explore a few of the variables that affect the Hill reaction and learn how the photo system works.Table 1:Experiment 1TimeTube01234510.55820.8150.5540.4040.2890.1630.0330.5590.1910.0080.0050.0020.00240.6030.5880.5810.5740.570.561Table 2Experiment 2Tube012345Intensity10.6290.3070.1830.1080.1080.1010.0120.5970.3140.1390.090.0640.0320.002530.4090.2170.10.0160.010.0080.00111
1-10-05Experiment 1: Graph 1Change in Absorbance Over Time00.10.20.30.184.108.40.206.80.9012345Time (min)Change in AbsorbanceTube 2Tube 3Tube 4Experiment 2: Graph 2Change in Absorbance vs. Time00.10.20.30.40.50.6012345Time (min.)Change in Absorbance10 cm 20 cm30 cm2