lab4D - than the pKa of the most acidic proton This ensures...

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Charles Ng 8222 Lab 4D Discussion Questions 1. An experiment to determine which ligates the iron would be a titration. You should change the glutamic acid to tyrosine, which is very different structurally. In the cause of iron ligation it is very specific. The enzyme will only show activity if it is ligated to the correct amino acid. If the enzyme is dysfunctional and does not show any or much activity, you know tyrosine is NOT the ligand. There should be high activity in the glutamic acid if it is the true ligand. 2. In order to determine whether an acid is fully protonated in solution the pH of the solution must not be higher
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Unformatted text preview: than the pKa of the most acidic proton. This ensures that the protons have not dissociated because the pKa of the most acidic (most likely to dissociated) proton has not yet been reached. In the case of the graph to the left, the first pKa (most acidic) is 2.2. In order to ensure the acid is fully protonated, the solution pH must not be higher than 2.2. This ensures that the protons that will dissociate at 2.2 will still be on the molecule. 3. a. b....
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This note was uploaded on 09/07/2011 for the course CHEM 272 taught by Professor Dr.brooks during the Spring '08 term at Maryland.

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lab4D - than the pKa of the most acidic proton This ensures...

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