TLC Lab Report - TLC Lab Report (09-Apr-2007) Norbert Wang...

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TLC Lab Report (09-Apr-2007) Norbert Wang GSI: N. Stephanopoulos 105 Purpose:  To learn how to use Thin Layer Chromatography (TLC) to identify components in an  unknown analgesic. Choosing the Solvents:  TLC tests a compound’s preference over a stationary or mobile phase.  The stationary phase is the solid silica gel plate and the mobile phase is the solvent. Compounds  spotted, based on their intermolecular forces with the silica or solvent, prefer either the silica or  solvent more. This preference is displayed in how far the compound traveled up the plate. A  compound with stronger intermolecular forces with the solvent usually meant that it moved  farther up the plate than a compound with weaker forces with the solvent. A good TLC solvent is  one that distributes the studied compounds well across the plate. The better distribution means  that each compound can be easily distinguishable from the others and therefore their Rf values  will be unique. Based on this measure, the solvents chosen were 100% Et Acetate and 10:10:1  Toluene: Et Acetate: Acetic Acid. 
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TLC Lab Report - TLC Lab Report (09-Apr-2007) Norbert Wang...

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