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3.1 - 1 genome is fragmented and a library is prepared 2...

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Chapter 3 I. Genomes are big and complex A. Technology does not allow us to see it all at once: 1. Analogy: Lewis and Clark mapped North America on foot, but today all North America can be photographed from a satellite B. Mapping Genome Concepts 1. denomes must be mapped as a prelude to obtaining a genome sequence 2. not simple: technology limitations, etc 3. human genome 3.75 x 10^9bp, sequencing capacity 800 bp/run= 4.69 million sequencing runs, 1 hr per run would take one sequencer 535 years to finish 4. complexity of genomes create problems, human genome is populated by enormous amounts of repetitive sequences C. Clone Contig Method
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Unformatted text preview: 1. genome is fragmented and a library is prepared 2. the fragments are positioned relative to one another base on the genetic markers they contain (mapped) 3. then the clones are sequenced to produces a contiguous sequence of the genome D. The whole genome shotgun approach 1. genome is fragmented and library is prepared 2. fragments ends are sequenced 3. a computer is used to identify overlaps and produce a contiguous sequence 4. works well for small simple prokaryotic genomes but is problematic for large and complex eukaryotic genomes 5. genome wide repeats creates a problem for this approach...
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