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3.2 - 3 human genome(haploid 3 trillion bp = 300,000 SNP...

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Chapter 3 continued A. Genomic Maps 1. genetic map – markers, positions defined by alleles and mapped through segregation analysis 2. physical map – means nucleotide sequence positions B. Genetic Markers 1. URA3 – a gene that encodes an enzyme for biosynthesis. When mutated grows only when uracil is present in cell media C. Polymorphic Restriction Site 1. RFLP analysis 2. digest, put on membrane, and then hybridize with a DNA probe D. SSLP : simple sequence length polymorphisms 1. microsatellite DNA - smallest, dispersed throughout genome, 13bp, more useful for genome mapping 2. minisatellite DNA – 25bp, typically concentrated around ends of chromosomes 3. PCR is the preferred way to analyze, which also works better on microsatellites, as with RFLPs 4. “Typing an SSLP by PCR”: means determining which allele an individual carries E. SNP: Single Nucleotide Polymorphism 1. SNP’s are very common is eukaryotic genomes 2. approximately 1 for every 10,000 bp
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Unformatted text preview: 3. human genome (haploid) 3 trillion bp = 300,000 SNP markers possible F. Detecting Hybridization by Dye – Quenching 1. oglionucleotide has a center that is complementary to the SNP of interest, when oglionucleotide doesn’t hybridize, there is no quenching and there will be no fluorescence G. DNA chips 1. allow many SNPs to be analyzed at once 2. high density chips can contain 300,000 oglionucleotides per c^2 allowing all humans SNPs to be analyzed in one experiment H. ARMS test 1. uses DNA polymerase that lacks 3-5 exo nuclease activity I. Linkage Analysis is Basis of Genetic Mapping 1. genes come in different versions called alleles 2. alleles segregate randomly and independently 3. crossing over during meosis exchanges alles between homologous chromosomes 4. the closer together the genes are the less likely crossing over will occur between them...
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