4.1 - B 3.29 3.30 3.32 3.33 IV Sequence Tag Site Mapping A...

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I. Genetic Map: (linkage analysis) vs Phsycial Map: (sequence) A. genetic map 1. relies on ability of DNA to cross over during meiosis, not uniform across the chromosome 2. cane only serve in the initial phase of a genome sequencing project 3. linkage analysis is determined in bacteria a. conjugation (predominant)- F plasmid encodes proteins and donates chromosome to recipient, uses a pillus, and mediates direct transfer. This transfer process begins at origin of transfer, allow us to map b. transformation c. transfection B. Physical map 1. restriction maps 2. fish maps 3. sequence tagged sites II. Methods A. R polymprphi crestriction site B. Nonpolymorphic restriction site III. Limitations of Restriction Mapping A. use specific enzymes that cut less frequently makes it more useful
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Unformatted text preview: B. 3.29, 3.30. 3.32, 3.33 IV. Sequence Tag Site Mapping A. any sequence can be used as a marker for mapping purposes B. random genomic sequences expressed sequence tags C. analyze a clone library to determine whether clones contain two STS D. the frequency that both STS are found on the same fragment is proportional to the distance apart they are E. end up with a large collection. F. The closer the STSs are, the more clones that will contain both. G. Can use them to make physical map, by randomly fragmenting the genome and looking at frequency of two tagged sites H. cDNA is derived from expressed genes. Therefore favored because the sequence tagged sites will be distributed as genes...
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This note was uploaded on 09/07/2011 for the course BIO 01:119:101 taught by Professor Marin during the Spring '08 term at Rutgers.

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