5.1 - o Methylation of DNA silences genes o DNA methyltransferases o%’-CG#’ or%’CNG-3” sequences o In higher eukaryotres 10 to 30 of

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Regulated sensitivey to DNase 1 has to do with gene regulation. It must be release from histones before it can be targeted Nucleosome Remodeling o Second type of chromatin modification that can influence genome activity o Is a change in the structure of nucelosomes that does not necessarily involve histone modification o Nuclesome structures is changed or displaced by sliding (cis-displacement) of removal ( trans) o Just as in histone modification, remodeling is carried out by protein complexes. Hsp70 is a heat shock protein o This process is not covalent Gene silencing by DNA modification
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Unformatted text preview: o Methylation of DNA silences genes o DNA methyltransferases o %’-CG-#’ or %’CNG-3” sequences o In higher eukaryotres 10 to 30% of cytosines o Maintenance and de novo DNA methlytransferases. Maintenance- recognizes hemi mehtylated strand and add methyl group to other C on the strand De novo- a additional methyl group is added to a strand that didn’t have it before. o De novo mehtlyation is needed for normal mammalian embryo development Dnmtl- maintance DNA mathylase Dwmt3a and dnmt3b de novo methylases (KO causes embryo death)...
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This note was uploaded on 09/07/2011 for the course BIO 01:119:101 taught by Professor Marin during the Spring '08 term at Rutgers.

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5.1 - o Methylation of DNA silences genes o DNA methyltransferases o%’-CG#’ or%’CNG-3” sequences o In higher eukaryotres 10 to 30 of

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