11.1 MG - Chapter 11 Assembly of Transcription Initiation...

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Unformatted text preview: Chapter 11 Assembly of Transcription Initiation Complex..continued • principles of DNA binding proteins interaction with dna binding site o Direct readout N-bases buried in the center of the double helix but are accessible from the outside o Contact b/t proteins and dna surface is with the phoshphodiester backbone via electrostatic interaction (non-specific integration, ex hisotnes) o Contact w/ N-base is formed thru H-bonds and hydrophobic interactions (can be specific interactions) o Specific interactions are determined by the number of contact sites, the larger the number the stronger/more stable the interaction. • H-bonds are oriented (rt) leucine zipper contact points maximized in a dimer configuration Future-omic Research goal o Predict the binding site of a protein based on its amino acid sequence o Progress is being made with zinc finger class o b/c in ZF proteins 4 amino acids in the helix and one at the base always interact with DNA predict with proteins will interact w/ nitrogenous bases ...
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This note was uploaded on 09/07/2011 for the course BIO 01:119:101 taught by Professor Marin during the Spring '08 term at Rutgers.

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11.1 MG - Chapter 11 Assembly of Transcription Initiation...

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