11.2 MG - by general transcription factor GTF made up of...

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RNA polymerases o Eukaryote forms > 500 kDa and 8-12 subunits (indirect attachment) o Bacterial RNAP 4 subunits one of them in two copies alpha 2, beta, beta’, sigma direct attachment o Indirect attachment of RNAP o
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o o basic initiation Expression of Genes can vary b/c sequence of promoter may differ from promoter. o Sequences determines strength of the promoter.
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Events of transcription initiation in bacteria o RNAP bind to -35 sequence (sigma subunit) o Open complex is formed at -10 (A-T rich) o Promoter clearance occurs upon initiation and subunit dissociates from the core enzyme (alpha 2 beta beta’) Eukaryotic RNAP binding site is defined by binding sties for proteins that makeup the platform o Core/basal promoter and upstream promoter elements. mo st RNA promoters contain TATATA sequence (RNAPII) Eu RNAP II o Most variable but most contain a TATA box centered -25 TATA is bound
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Unformatted text preview: by general transcription factor GTF made up of TFIID, TBP (tata binding protein), and 12 TAFs o Initiator sequence defines the initiation site o DPR downstream promoter element o GC_GC rish element bound by TFIIB o PSE-Proximal sequence element Events in RNAP II o RNAP II must bind to various proteins in promoter But be phospholiated at CTD (located in largest subunit) to begin transcription (from ATP) However RNAP must be bound at first 2 Proteins bind it to the promoter TFIIF, TFIIB TFIIH catalyzes unwinding of the DNA (has helicase activity) initiates transcription TFIIEinitiates transcription TBP binds at the minor groove and sites like a saddle One of the TAF;s has a histone-like fold pseudonucleosome...
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11.2 MG - by general transcription factor GTF made up of...

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