Unformatted text preview: E. only two aa on receptor contribute most of E for binding the hormone 2. ligation of 1 receptor often leads to binding of 2 nd receptor = dimerization 3. dissociation constant Kd is a measure of the affinity of R for L . kd=k off /k on = [R][L]/[RL]. Tight binding = lower koff & kd 4. in a binding assay, kd corresponds to conc of ligand at which 50% of receptors are bound 5. competition assay – detects kd of low affinity ligands 6. not all receptors need to be occupied to induce maximal response. Ex: 18% receptors induces ½ max of physiological response...
View Full Document
- Spring '11
- Cell Signaling, Ligand Receptor Interactions