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Unformatted text preview: E. only two aa on receptor contribute most of E for binding the hormone 2. ligation of 1 receptor often leads to binding of 2 nd receptor = dimerization 3. dissociation constant Kd is a measure of the affinity of R for L . kd=k off /k on = [R][L]/[RL]. Tight binding = lower koff & kd 4. in a binding assay, kd corresponds to conc of ligand at which 50% of receptors are bound 5. competition assay detects kd of low affinity ligands 6. not all receptors need to be occupied to induce maximal response. Ex: 18% receptors induces max of physiological response...
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This note was uploaded on 09/08/2011 for the course CELL BIO & 01:146:473 taught by Professor Brewer during the Spring '11 term at Rutgers.
- Spring '11