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ST 1 - E only two aa on receptor contribute most of E for...

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Signal Transduction Signal Transduction: Chapter 15.1-.4 I. Types of Signals A. Endocrine: long distance, several meters B. Paracrine: micrometers, targets neighboring cell C. Autocrine: a slap on your face, targets same cell D. Ligands: can trigger signaling in adjacent target cell, adherence molecules such as integrins II. Surface Receptors: all activated by ligand binding to receptor and usually leads to gene transcription A. Ligand Receptor Interactions: Harmonic Groove. Fig 15-3 1. essential residues on ligand and receptor determine binding specificity. Ex: 8/28 aa of hormone contribute to 85% of binding
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Unformatted text preview: E. only two aa on receptor contribute most of E for binding the hormone 2. ligation of 1 receptor often leads to binding of 2 nd receptor = dimerization 3. dissociation constant Kd is a measure of the affinity of R for L . kd=k off /k on = [R][L]/[RL]. Tight binding = lower koff & kd 4. in a binding assay, kd corresponds to conc of ligand at which 50% of receptors are bound 5. competition assay – detects kd of low affinity ligands 6. not all receptors need to be occupied to induce maximal response. Ex: 18% receptors induces ½ max of physiological response...
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