Cell Cycle 2 - A transition(chromosome separation and A to...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Specificity of CDK MPF Cdc25 Ubiquitin Ligases APC/C SCF ligase Proteolutic removal of CDKs and cell cycle mediators Sea Urchin Exp G1 cyclin CDKs S phase cyclin CDKs SiC1 can bind to multiple cyclins. Cyclin determines substrate specificity. Mitosis Promoting Factor, a CDK, phosphorylates >100 specific proteins to trigger M phase entry (chrom condensation, MT remodeling, disassembly of nuc membrane into ER) Aka: CDK1, CDC2, p52 kinase 2 steps - Yeast: Activated by dephosphorylation of P~Y15 inhibitory site and phosphorylation of T161 activating site, humans: binding of cyclin A, phosphorylation of T160 The phosphatase that activates MPF by removing its inhibitory phosphate Regulate cell cycle, drive it forward Anaphase Promoting Complex/Cyclosome; induces M to
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: A transition (chromosome separation), and A to T transition. An E3 ubiquitin ligase. Controlled by two different specificity factors: Cdc20 or Cdh1 (activated when one of these binds). High level throughout cell cycle. A ubiquitin ligase that regulates transition for G1 to S, is a protein complex. High level throughout cell cycle Is irreversible, so the cells has to move forward toward division Revealed cyclin protein and that its levels changed during the cell cycle Set up cells for S phase Turn on ORIs. In yeast, degradation of Sphase inhibitor triggers DNA replication. Accumulate during G1, but are inhibited Sphase cyclin inhibitor that becomes hyperphosphorylated as G1 cyclins build up in cell, and undergoes ubiquitination by SCF ligase...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern