Cell Cycle 2 - A transition(chromosome separation and A to...

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Specificity of CDK MPF Cdc25 Ubiquitin Ligases APC/C SCF ligase Proteolutic removal of CDKs and cell cycle mediators Sea Urchin Exp G1 cyclin CDKs S phase cyclin CDKs SiC1 can bind to multiple cyclins. Cyclin determines substrate specificity. Mitosis Promoting Factor, a CDK, phosphorylates >100 specific proteins to trigger M phase entry (chrom condensation, MT remodeling, disassembly of nuc membrane into ER) Aka: CDK1, CDC2, p52 kinase 2 steps - Yeast: Activated by dephosphorylation of P~Y15 inhibitory site and phosphorylation of T161 activating site, humans: binding of cyclin A, phosphorylation of T160 The phosphatase that activates MPF by removing its inhibitory phosphate Regulate cell cycle, drive it forward Anaphase Promoting Complex/Cyclosome; induces M to
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Unformatted text preview: A transition (chromosome separation), and A to T transition. An E3 ubiquitin ligase. Controlled by two different specificity factors: Cdc20 or Cdh1 (activated when one of these binds). High level throughout cell cycle. A ubiquitin ligase that regulates transition for G1 to S, is a protein complex. High level throughout cell cycle Is irreversible, so the cells has to move forward toward division Revealed cyclin protein and that its levels changed during the cell cycle Set up cells for S phase Turn on ORIs. In yeast, degradation of Sphase inhibitor triggers DNA replication. Accumulate during G1, but are inhibited Sphase cyclin inhibitor that becomes hyperphosphorylated as G1 cyclins build up in cell, and undergoes ubiquitination by SCF ligase...
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This note was uploaded on 09/08/2011 for the course CELL BIO & 01:146:473 taught by Professor Brewer during the Spring '11 term at Rutgers.

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