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DNA transcription 1 - b 3’ OH Comes from H-bonding(2x...

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Nucleotides purines pyrimidines organic bases nucleoside mononucleotide diphosphate nucleotide phosphoric acid phosphodiester bond specificity of base pairing stacking interactions 3D structure Grooves Tm of DNA Transcription initation requirements Initation Elongation Termination A phosphate group, pentose sugar, organic base Adenine and guanine – two fused rings Uracil, thymine, cytosine – one ring Heterocyclic, conatin N that is not ionized at physiological pH, planar Sugar + base Single phosphate on 5’ Two phosphates on 5’ Ionizes at physiological pH, gives nucleic acids their acid character Condense between α and β phosphates of a triphosphate with 3’OH (alcohol + acid = ester) direction 5’ has P
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Unformatted text preview: b 3’ OH Comes from H-bonding (2x distance of covalent bond), Bases have hydrophobic interactions, with e clouds above & below plane, strengthen dbl helix 10 bp/turn, 3.4 nm/turn Most proteins bind DNA in major groove Affected by G+C content, solvent, nature of DNA Requires DNA-dependent RNA polymerase (RNA polymerase), promoter, transcription factors, nucleotide triphosphates RNA polymerase Binds to DNA promoter, melts DNA near start site to form transcription bubble/open complex, catalyzes phosphodiester bonds Polymerase reads 3’-5’, synthesizes 5’-3’ Releases RNA and dissociates from DNA...
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