Unformatted text preview: keep pigments pet ether * keep Add 25 mL pet ether; Place 1 mL of pigment mixture gently swirl in the sep funnel; in 125 mL sep. funnel; allow the two liq layers to separate; Add 20 mL of 80% acetone draw off lower layer discard pigments Add 25 mL H2O; pet ether gently swirl; draw off lower layer discard H 2O Circle the best answer acetone H 2O pet ether is (more, less) polar than methanol. chlorophyll a and carotene are (more, less) polar than chlorophyll b and xanthophyll
keep repeat washing w/ 25 mL H2O two more times; draw off lower layer each time discard pigments Add 25mL 95% methanol; pet ether gently swirl; draw off lower layer keep upper pet ether layer contains chlorophyll a and carotene A. * keep lower methanol layer contains chlorophyll b and xanthophyll B. H 2O keep Slowly add 10 mL of 30% KOH sol.; gently shake for 10 minutes; A. allow layers to separate; top layer should be yellow. keep upper yellow pigment the upper layer contains pet ether layer the upper pet ether layer is (green, yellow)? (chlorophyll a, carotene)
the lower methanol/H2O layer is (green, yellow)?
lower pigment methanol/H 2O layer the lower layer contains keep Slowly add 10 mL of 30% KOH solution; gently shake for 10 minutes; allow layers to separate (chlorophyll a, carotene) diethyl ether is (more, less) polar than methanol. chlorophyll b and xanthophyl are (more, less) polar than methanol.
keep Place methanol layer back into the sep funnel; add 25 mL diethyl ether; B. gently swirl; add 5 mL portions of water; swirl after each addition until discard layers separate (about 15 mL H2O) pigments diethyl ether upper pigment diethyl ether layer the upper layer contains the upper diethyl ether layer is (green, yellow)? (chlorophyll b, xanthophyl)
the lower methanolic KOH layer is
keep lower pigment (green, yellow)? methanol/H 2O layer methanol H 2O the lower layer contains (chlorophyll b, xanthophyl) ...
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- Spring '11
- Organic chemistry, Solvent, Diethyl ether, Purple