plant_pigment_new_exp

plant_pigment_new_exp - Separation and Identification of...

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1 Separation and Identification of Plant Pigments Dr. Gergens - SD Mesa College PURPOSE In this experiment, the photosynthetic pigments common to all flowering plants will be extracted by liquid- liquid extraction. The four main pigment components of plant leaves are chlorophyll a, chlorophyll b, carotene, and xanthophyll. The solvents needed for the extraction and separation will be petroleum ether, diethyl ether, methanol, and water. Finally, these pigments will be analyzed by measuring the absorption spectra of the plant pigments. BACKGROUND The four main pigment components of plant leaves are chlorophyll a, chlorophyll b, carotene, and xanthophyll. While most plant leaves appear green to our eyes, several different photosynthetic pigments of various colors are usually present in the chloroplast of green plants. The colors of these pigments are due to the numerous double bonds in their structure. Chlorophyll a and b provide the green color of leaves, which absorb most of the light energy needed for photosynthesis to occur. The greatest absorption of light by chlorophyll is in the red, blue and violet wavelengths. Associated with these green pigments are several other pigments known as carotenoids. These are commonly yellow to red in color, and are also involved in harvesting light energy for photosynthesis.
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2 RESULTS/DISCUSSION (include the following in your formal conclusion) 1. Include graphs of the four spectra for chlorophylls a and b, carotene and xanthophyll 1. Include structures for each pigment. Paste the structure of each compound into the appropriate cell. If necessary do the following <Insert>, <Paste Special>, <Picture> and turn off [float over text]
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plant_pigment_new_exp - Separation and Identification of...

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