bio web quiz 17

bio web quiz 17 - 1. During gel electrophoresis smaller...

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1. During gel electrophoresis smaller sections of DNA move further through a gel than larger sections of DNA with fewer bases the smaller sections form fewer base-pairs with the agarose. smaller sections of DNA have a higher charge-to-mass ratio. the gel matrix resists the movement of smaller items less than larger ones. the larger sections of DNA are slightly attracted to the negative pole of the gel. smaller sections are single-stranded, and so move faster. 2. You digest a piece of DNA with two different restriction endonucleases and set up three reactions: two reactions get a single enzyme, the third reaction receives both. You run these reactions on an electrophoresis gel. Well 1 gets the reaction with both enzymes, wells 2 and 3 receive the reactions with single enzymes. The figure to the right shows the results. Which of the following correctly describes the location of the positive electrode and shows a valid relationship between the fragment sizes in the bands (A-E)? + electrodes on left, C = A + E
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bio web quiz 17 - 1. During gel electrophoresis smaller...

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