unit7 cloning animals

unit7 cloning animals - UNIT 7 Animal Cloning and...

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1 UNIT 7 Animal Cloning and Epigenetics F08 The first cloned horse. What cell parts would you need to clone a human? (or a horse)
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2 Exam #3 TU 12/02 Unit 6 DNA Forensics TH 12/04 Unit 7 Human Cloning TU 12/09 In class review for Exam #3 TH 12/11 EXAM #3 in class FR 12/12 last day of classes at UMass Three (3) in-class exams: NO cumulative final exam! TU 10/07 EXAM #1 TH 11/06 EXAM #2 TH 12/11 EXAM #3 Each exam is worth 1/3 of final course grade.
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3 A different perspective on human cloning…
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4 Human Cloning! Somatic Cell Nuclear Transfer (SCNT) Donor nucleus from the person to be cloned Nucleus is removed from an egg cell Donated nucleus is transferred into an empty egg cell (no nucleus) 1) 3) 2) Nucleus from recipient egg is removed and discarded. “Cloned” zygote divides… 4) What happens to cloned zygote?
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5 Need a donor nucleus (person to be cloned) and empty egg Donor nucleus from “person” to be cloned Remove the from a recipient egg cell Transfer donated nucleus into an enucleated egg cell (lacks a nucleus) “Cloned” zygote begins to divide… 4) Unfertilized egg Nucleus removed from egg cell Empty egg (incubator for new nucleus!) Egg nucleus is discarded
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6 Donor nucleus is transferred into empty egg cell Remove the from a recipient egg cell Transfer donated into an egg cell (lacks a nucleus) “Cloned” zygote begins to divide… Empty cell discarded Nucleus from egg cell is discarded. Recipient egg receives new nucleus – the “cloned” egg cell begins to develop Donor nucleus (from person to be cloned) is transferred into the empty egg cell Empty egg (incubator for new nucleus!)
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7 Donor Nucleus Transfers Donor Genome to Recipient Cell whyfiles.org/148clone_clash/2.html 1) Nucleus is removed from cytoplasm of an egg cell. 2) Syringe needle picks up a single donor nucleus 3) “Cloned” nucleus is injected into the cytoplasm of the empty egg cell. Single nucleus Holding pipette Transfer needle Egg cell Nucleus Egg cell
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8 Grow to blastocyst and implant in uterus (pregnancy) “Cloned” blastocyst “Cloned” blastocyst can be: implanted in surrogate Mom or harvested for ESCs (ICM) Egg contains donor nucleus In animal cloning, the blastocyst is implanted into the uterine wall of the surrogate mother: called reproductive cloning . “Cloned” zygote
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9 Two main applications of Human Cloning Major Applications of Human Cloning: Reproductive Cloning: creates a human being who is genetically identical to the person being cloned 2) Therapeutic Cloning: creates stem cells with a pre-determined genetic makeup for therapeutic use (also genetic match for transplant)
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Dolly: Reproductive cloning by SCNT Donor nucleus removed from the skin cell of an adult sheep was transferred into the empty cytoplasm of a sheep egg cell. In the egg cytoplasm, the donated adult sheep genome undergoes “nuclear reprogramming”, which directs the cell to become less specialized and form a blastocyst embryo. The blastocyst is implanted into a surrogate female sheep which becomes pregnant. Dolly the lamb was a genetic clone of the adult sheep that donated the skin cell nucleus.
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unit7 cloning animals - UNIT 7 Animal Cloning and...

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