ch-20-guided-reading

ch-20-guided-reading - Adapted from L. Miriello by S. Sharp...

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Adapted from L. Miriello by S. Sharp AP Biology Name _________________________ Chapter 20 Guided Reading Assignment 1. Define the following a. Recombinant DNA b. Genetic engineering c. Biotechnology d. Gene cloning 2. Why did restriction enzymes evolve in bacteria? 3. Define the following terms a. Restriction site b. Restriction fragments c. Sticky end 4. Label the following diagram. 5. Using the diagram below – label the steps to cloning a human gene in a bacterial plasmid Page 1 of 8
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Adapted from L. Miriello by S. Sharp 6. Explain in your own words two ways that we know the cell clones carry the recombinant plasmids? 7. What is the purpose of nucleic acid hybridization? Why is the word hybrid used? 8. What is a complementary, short, single stranded nucleic acid that can be either DNA or RNA called? 9. Why do scientists use a radioactive isotope tag for the probes? Page 2 of 8
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Adapted from L. Miriello by S. Sharp 10. How is DNA denaturation different than protein denaturation? 11.Label the following steps of nucleic acid probe hybridization.
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This note was uploaded on 09/09/2011 for the course BIO 101 taught by Professor Harounal-rashed during the Spring '11 term at King's College London.

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ch-20-guided-reading - Adapted from L. Miriello by S. Sharp...

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