Lab 1.docx - Name Lane Evers Section_100 LAB 1 Measurement...

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Name: Lane Evers___ Section:_100_________ LAB 1: Measurement, Pipette Use, and the Standard Curve Experiment 2 Standard Curve Table 1 Tube Final Protein Concentration µL of BSA protein stock (0.5 mg/mL) needed Bradford Reagent 0.15M NaCl for 1.0 mL final volume 8 50 µg/mL 0.1 mL or 100 µL 900 µL 0 mL 7 25 µg/mL 0.05 mL or 50 µL 900 µL 0.05 mL 6 15 µg/mL 0.03 mL or 30 µL 900 µL 0.07 mL 5 10 µg/mL 0.02 mL or 20 µL 900 µL 0.08 mL 4 7.5 µg/mL 0.015 mL or 15 µL 900 µL 0.085 mL 3 5.0 µg/mL 0.01 mL or 10 µL 900 µL 0.09 mL 2 2.5 µg/mL 0.005 mL or 5 µL 900 µL 0.095 mL 1 0 µg/mL 0 mL or 0 µL 900 µL 0.1 mL Table 2 Final Concentration Absorbance 595nm 50 µg/mL 0.64 25 µg/mL 0.39 15 µg/mL 0.47 10 µg/mL 0.24 7.5 µg/mL 0.12 5.0 µg/mL 0.05 2.5 µg/mL 0.01 0 µg/mL 0 Part B: Protein Concentration in Milk Table 3 My Milk Type = Soy Milk The Dilution of milk I used was: 1:100 Tube Unknown Volume Bradford Reagent 0.15M NaCl for 1.0 mL final A 595nm Concentration Measured by Spec. Concentration of original milk 1 5 µL 900 µL 95 µL 0.01 0.388 µg/mL 7.76 mg/mL 2 10 µL 900 µL 90 µL 0.05 1.938 µg/mL 19.38 mg/mL 3 20 µL 900 µL 80 µL . 0.1 3.876 µg/mL 19.38 mg/mL 4 40 µL 900 µL 60 µL 0.18 6.977 µg/mL 17.493 mg/mL

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