Lecture 07 Control of Transcription

Lecture 07 Control of Transcription - Describe the...

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Unformatted text preview: Describe the structure of a gene and learn terminology Recognize that regulation of gene expression is a primary mechanism underlying reproduction, development, homeostasis, and immunity Describe the process of transcription Describe the roles of chromatin remodeling factors and covalent modifications of histones Describe the general steps in the initiation, termination, and processing of mRNA Vocab Promoter, enhancer, nucleosome, chromatin,histone modification, chromatin remodeling, mRNA, RNA polymerase II, general transcription factors, DNA binding domain, DNA methylation, epigenetic, imprinting Outline: • Organization of genes • Chromatin structure and histone modifications • RNAs and polymerases • “core” transcriptional machinery • DNA regulatory elements • Sequence – specific transcription factors • Transcriptional mechanisms establishing cellular identity and function • DNA methylation and transcription- Why is this topic medically important? (examples) o Transcription factors contribute to disease Estrogen receptors breast cancer Androgen receptors prostate cancer o Mutations in DNA binding sites for transcription factors contribut disease Most polymorphisms linked to disease are not in protein-codi regions of the genome (mutation in region of DNA is not at th gene region but the regions of DNA that control the expressio replication of the gene) o Forced expression of transcription factors enables reprogrammin patient fibroblasts to different cell types Applications to regenerative medicine (cell to the stem cell – can reprogram)- Genome organization at different powers of ten o Gene separated by exon/intron –intron will spliced off to turn on/off o Chromatin is the template for transcription The challenge here is to be able to transcribe in presence of chromatin w/ histones • The beads on a string – is what transcription deal with • The other condense forms are more for cell division The nucleosome is the basic unit of chromatin (the histone move around the nucleosome Describe the structure of a gene and learn terminology Recognize that regulation of gene expression is a primary mechanism underlying reproduction, development, homeostasis, and immunity Describe the process of transcription Describe the roles of chromatin remodeling factors and covalent modifications of histones Describe the general steps in the initiation, termination, and processing of mRNA Describe the role of different types of DNA regulatory elements Vocab Promoter, enhancer, nucleosome, chromatin,histone modification, chromatin remodeling, mRNA, RNA polymerase II, general transcription factors, DNA binding domain, DNA methylation, epigenetic, imprinting Outline: • Organization of ge n e s • Chrom atin structure a nd histone m odifications • RNAs a nd polym eras es • “core” transcriptional m ac hinery • DNA regulatory ele m e nts • Seque nc e – sp ecific transcription factors •...
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This note was uploaded on 09/14/2011 for the course PHARM HB taught by Professor Staff during the Spring '11 term at UCSD.

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Lecture 07 Control of Transcription - Describe the...

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