Lecture 11 RNA Processing and MicroRNAs

Lecture 11 RNA Processing and MicroRNAs - Describe mRNA...

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Unformatted text preview: Describe mRNA splicing, including an example in which defects leads to disease Explain the role of alternative mRNA splicing in increasing the coding potential of the genome Describe polyadenylation of mRNA and explain its effects on mRNA stability, mRNA transport, and formation of the translation complex Describe the biogenesis and function of human micro RNAs (miRNAs) Describe how miRNAs act as part of the RNA-inducing silencing complex (RISC) Explain how cellular miRNAs can affect the expression of viral mRNAs and cancer mRNAs Describe the potential benefits and challenges of using miRNA as a therapeutic agent Vocab: 5’ end capping of mRNA, polyadenylation (CstF, CPSF, Poly A Polymerase, Poly A Binding Proteins); RNA splicing, Intron, Exon, spliceosome, small nuclear RNAs (snRNAs) and small nuclear RNA proteins (snRNPs); RNA Polymerase carboxy terminal domain (CTD); microRNA (miRNA), short- interfering RNA (siRNA); DICER; DROSHA; RISC; RITS; Fragile X Mental Retardation Protein (FMRP); Oncomirs; Anatgomirs; RNA editing (ADAR) Outline: • Capping 5’ end of RNA • Cleavage and polyadenylation of 3’ end of RNA • Mechanisms of splicing • Alternative splicing • miRNA • RNA editing- RNA Processing o Three m ajor process es 5’ capping Polyadenylation Splicing - 5’ capping o Process A GTP is attach ed to the 5’ e nd via a 5-5’ bond (enzym e = gu anyltransferas e) First few NT (including GTP at N # 7) are m ethylated (enzym e = gu anine-m ethyltransferase) o Effects Facilitates splicing Stabilizes the mRNA transcript Facilitates transport of mRNA to cytoplas m Recognition by riboso m es in translation Describe mRNA splicing, including an example in which defects leads to disease Explain the role of alternative mRNA splicing in increasing the coding potential of the Describe polyadenylation of mRNA and explain its effects on mRNA stability, mRNA transport, and formation of Describe the biogenesis and function of human micro RNAs Describe how miRNAs act as part of the RNA-inducing silencing complex (RISC) Explain how cellular miRNAs can affect the expression of viral mRNAs and cancer Describe the potential benefits and challenges of using miRNA as a therapeutic Vocab: 5’ end capping of mRNA, polyadenylation (CstF, CPSF, Poly A Polymerase, Poly A Binding Proteins); RNA splicing, Intron, Exon, spliceosome, small nuclear RNAs (snRNAs) and small nuclear RNA proteins (snRNPs); RNA Polymerase carboxy terminal domain (CTD); microRNA (miRNA), short-interfering RNA (siRNA); DICER; DROSHA; RISC; RITS; Fragile X Mental Retardation Outline: • Capping 5’ end of RNA • Cleavage and polyadenylation of 3’ end of RNA • Mechanisms of splicing • Alternative splicing • miRNA • RNA editing- Polyadenylation o Addition of 40-250 untemplated adenosine nucleotides to...
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This note was uploaded on 09/14/2011 for the course PHARM HB taught by Professor Staff during the Spring '11 term at UCSD.

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Lecture 11 RNA Processing and MicroRNAs - Describe mRNA...

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