Lecture 12 Protein Synthesis, Modification, and Degradation

Lecture 12 Protein - Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA

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Unformatted text preview: Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA into protein Define several types of point mutations, including silent mutations, missense mutations, nonsense mutations, frameshift mutations, and splicing mutations, and describe their effects on protein products Vocab: Codon, anticodon, wobble, aminacyl tRNA synthetase, eIF4 Cap-Binding complex, eiF2 initiation complex, ternary complex, kozak sequence, initiator tRNA, ribosome A site, P site, E site, elongation factors, peptidyl transferase reaction, peptide bond formation, aminoglycosides, , cycloheximide, missense, nonsense, and frameshift mutations, internal ribosome entery sites (IRES), ubiquitin – proteasome-mediated degradation, molecular chaperomes, bortezomib Outline: The big picture Genetic code Structure and function of tRNA Addition of amino acids to the tRNA Structure and function of ribosoes Steps in protein synthesis Regulation of translation Therapeutics – inhibition of translation Viral exploitation of the translational apparatus Post-translational processing of proteins The ubiquitin and proteasome mediated degradation pathway Drug targeting and the proteasome-- The big picture o Central dogma: DNA mRNA Protein o Translation = process by which mRNA is translated to a polypeptide chain (second stage); protein synthesis o Translation is the basis of how our genetic code translated into cellular, biochemical, and physiologic functions o Without translation of the mRNA intermediate into protein, life could not exist- The important ingredients of translation (whats required for translation) o Mature mRNA in cytoplasm o tRNA’s each charged with an amino acid o polyribosomes , which are protein synthesizing factories o GTP and ATP as sources of energy o Ribosome complex is the 50S for the big and 40S for the small- Language of the Genetic Code o Triple code – a codon is the t rinucleotide sequence that codes for an amino acid 4^3 = 64 61 amino acids, 3 termination codons o Degeneration – multiple codon 1 AA o Redundant – some amino acids have more than 1 tRNA that can be charged with the amino acid o Wobble: a phenomenon that allows the 5’ end of the anticodon to hybridize to several different nucleotides at the 3’ end of the codon Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA into protein Define several types of point mutations, including silent mutations, missense mutations, nonsense mutations, frameshift mutations, and splicing mutations, and describe their effects on protein products Vocab: Codon, anticodon, wobble, aminacyl tRNA synthetase, eIF4 Cap-Binding complex, eiF2 initiation complex, ternary complex, kozak sequence, initiator tRNA, ribosome A site, P site, E site, elongation factors, peptidyl transferase reaction, peptide bond formation, aminoglycosides, , cycloheximide,...
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This note was uploaded on 09/14/2011 for the course PHARM HB taught by Professor Staff during the Spring '11 term at UCSD.

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Lecture 12 Protein - Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA

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