{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 12 Protein Synthesis, Modification, and Degradation

Lecture 12 Protein Synthesis, Modification, and Degradation...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA into protein Define several types of point mutations, including silent mutations, missense mutations, nonsense mutations, frameshift mutations, and splicing mutations, and describe their effects on protein products Vocab: Codon, anticodon, wobble, aminacyl tRNA synthetase, eIF4 Cap-Binding complex, eiF2 initiation complex, ternary complex, kozak sequence, initiator tRNA, ribosome A site, P site, E site, elongation factors, peptidyl transferase reaction, peptide bond formation, aminoglycosides, chloramphenicol, cycloheximide, missense, nonsense, and frameshift mutations, internal ribosome entery sites (IRES), ubiquitin – proteasome-mediated degradation, molecular chaperomes, bortezomib Outline: The big picture Genetic code Structure and function of tRNA Addition of amino acids to the tRNA Structure and function of ribosoes Steps in protein synthesis Regulation of translation Therapeutics – inhibition of translation Viral exploitation of the translational apparatus Post-translational processing of proteins The ubiquitin and proteasome mediated degradation pathway Drug targeting and the proteasome - - The big picture o Central dogma: DNA mRNA Protein o Translation = process by which mRNA is translated to a polypeptide chain (second stage); protein synthesis o Translation is the basis of how our genetic code translated into cellular, biochemical, and physiologic functions o Without translation of the mRNA intermediate into protein, life could not exist - The important ingredients of translation (whats required for translation) o Mature mRNA in cytoplasm o tRNA’s each charged with an amino acid o polyribosomes , which are protein synthesizing factories o GTP and ATP as sources of energy o Ribosome complex is the 50S for the big and 40S for the small - Language of the Genetic Code o Triple code – a codon is the trinucleotide sequence that codes for an amino acid 4^3 = 64 61 amino acids, 3 termination codons o Degeneration – multiple codon 1 AA o Redundant – some amino acids have more than 1 tRNA that can be charged with the amino acid o Wobble: a phenomenon that allows the 5’ end of the anticodon to hybridize to several different nucleotides at the 3’ end of the codon Explain the function of tRNA in the process of protein synthesis Describe the function of the ribosome in translation of mRNA into protein
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Define several types of point mutations, including silent mutations, missense mutations, nonsense mutations, frameshift mutations, and splicing mutations, and describe their effects on protein products Vocab: Codon, anticodon, wobble, aminacyl tRNA synthetase, eIF4 Cap-Binding complex, eiF2 initiation complex, ternary complex, kozak sequence, initiator tRNA, ribosome A site, P site, E site, elongation factors, peptidyl transferase reaction, peptide bond formation, aminoglycosides, chloramphenicol, cycloheximide, missense, nonsense, and frameshift mutations, internal
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern