Lecture 17 Intracellular Transport - Protein Trageting to Cell Organelles

Lecture 17 Intracellular Transport - Protein Trageting to Cell Organelles

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Unformatted text preview: Name and describe the membrane-enclosed organelles that receive proteins via intracellular transport from the cytoplasm, including peroxisomes, mitochondria, endoplasmic reticulum, nucleus, Golgi, and lysosomes List, and describe the salient features of the three major protein import pathways: transport through nuclear pores (gated transport), transport across membranes (transmembrane transport), and transport by vesicles (vesicular transport) Describe the function of the key components of each of the protein import pathways for peroxisomes, mitochondria, the nucleus and the endoplasmic reticulum, including the sorting signals, receptors, translocators, and signal peptidases Describe the structure of the endoplasmic reticulum (ER) and its physical relationship to the nuclear membrane Describe the roles of the rough ER in protein synthesis and protein translocation Vocab: Targeting signal or signal sequence, transport receptor, protein translocator, signal peptidase, nuclear localization signal (NLS), Nucleoporins, Ran GTPase, signal recoginition particle (SRP), SRP receptor, start-transfer sequence, stop transfer sequence Outline:- General protein sorting mechanisms and pathways- Peroxisome protein import, function and assembly- Targeting and protein import into mitochondria- Gated transport or protein and RNA in and out of the nucleus- Targeting and translocation of proteins into the ER- Membrane enclosed organelles import proteins by one of three mechanisms o Protein synthesized in cytosol, and transport to elsewhere o Zip code, encoded protein nuclear pore, across membrane, transport to vesicle - Key components to protein targeting pathways o Signal sequence or zip code: amino acid sequence that directs protein to correct compartment o Transport receptor: binds signal, located on target organelle or in the cyytosol o Translocator: transport machinery in target membrane o Signal peptidase: removes signal sequence (optional)- Signal sequences direct proteins o the correct organelles o- Some typical signal sequences o- Structure and content of peroxisomes (liver) o Contain >50 enzymes Catalase, d-amino acid oxidase, urate oxidase (crystals)- Functions of the peroxisomes o Breakdown of fatty acids: beta-oxidation of long chain fatty acids o Detoxification of toxic molevule (ethanol, H2O2) o Synthesis of plasmologens (myelin, caridiolin) Name and describe the membrane-enclosed organelles that receive proteins via intracellular transport from the cytoplasm, including peroxisomes, mitochondria, endoplasmic reticulum, nucleus, Golgi, and lysosomes List, and describe the salient features of the three major protein import pathways: transport through nuclear pores (gated transport), transport across membranes (transmembrane transport), and transport by vesicles (vesicular transport) Describe the function of the key components of each of the protein import pathways for peroxisomes, mitochondria, the nucleus and the endoplasmic reticulum, including the sorting signals, receptors,...
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This note was uploaded on 09/14/2011 for the course PHARM HB taught by Professor Staff during the Spring '11 term at UCSD.

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Lecture 17 Intracellular Transport - Protein Trageting to Cell Organelles

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