Graph 3 - Bio 212L sec 10 Time(s Normal Extract Boiled...

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Bio 212L sec. 10 Time (s) Normal Extract Boiled Extract 20 0.198 0.019 40 0.372 0.033 60 0.596 0.054 80 0.812 0.095 100 1.022 0.12 120 1.222 0.142 Boiled Peroxidase Results (@ 470 nm) Questions: 1. The temperature that the enzyme is most active is at room temp. (25 C) and quite active between 25 C and 45 C. However, the enzyme is least effective near freezing (4 C) and boiling temperatures. 2. The temperature has outstanding biological significance on our natural world when it comes to enzyme affectivity. For example, the reason plants generally start to “spring” in the Spring is because that is when the temperature is enough above freezing point for the enzymes to be active, and also why in the middle of the summer, when it gets too hot, they die. 3. Boiling our solution was very damaging to our enzyme and as a result, the enzyme was much less effective than when it was at room temperature. It was even more damaging than freezing it. 4.
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This note was uploaded on 09/14/2011 for the course BIO 212 taught by Professor Mayfield during the Spring '10 term at Iowa State.

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Graph 3 - Bio 212L sec 10 Time(s Normal Extract Boiled...

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