c. elegans developmental biology

c. elegans developmental biology - C. elegans P Granule...

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C. elegans P Granule Paper It was once thought that P granules were essential during mitosis to distinguish germline from soma in C. elegans. The paper “Cytoplasmic Partitioning of P Granule Components Is Not Required to Specify the Germline in C. elegans” goes through the steps used to come to the conclusion that P granules are not required to distinguish the germline. Green florescence protein (GFP) was used to monitor live embryos expressing the p granule, the granules were stained with GFP and were observed very closely. Observations were made in regards to the p granules shrinking in the anterior and growing in the posterior during interphase. By the end of interphase 85% of the p granules in the anterior disappeared completely or crossed over to the posterior while the number of P granules increased. Florescence were observed equally in the anterior and posterior halves of the zygote during interphase, this showed that the P granule protein was still present in the anterior cytoplasm although P granules were not seen. It was once thought that a local change in concentration threshold for granule assembly was
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c. elegans developmental biology - C. elegans P Granule...

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