MSE2020LectureNotes_2_3

MSE2020LectureNotes_2_3 - MSE 2020 Page 1 of 6 Presentation...

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MSE 2020 2/3/2011 Page 1 of 6 Presentation: Confocal Microscopy Marvin Minsky patented 1 st confocal microscope – he’s currently a professor at MIT Based on the ideas of the fluorescence microscope o Its dichroic mirror only let 1 wavelength of light reach the sample o Issue: limited the depth of view, made the image out of focus Solution: confocal microscope Pinhole allows only focused light through to the detector o greater depth of view o light focused on a single point Average image speed – 3 pixels/sec (up to 30 pixels/sec with an acoustic confocal microscope) Acoustic: sound on a crystal instead of using light A confocal microscope can be an attachment to an existing machine Prototype developed in 1955, patented in 1961 Modernization o Laser as a light source (instead of original carbon arc) Damage to the sample is reduced by choosing the specific frequency needed o Added moving mirrors/acoustics (instead of original moving stages) o Computers eliminated the need to put the image on film
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This note was uploaded on 09/12/2011 for the course MSE 2020 taught by Professor Alamgir during the Spring '09 term at Georgia Tech.

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MSE2020LectureNotes_2_3 - MSE 2020 Page 1 of 6 Presentation...

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