Lecture3 - MCB 110L Lecture 3 1/26/2011 Qing Zhong 306...

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Unformatted text preview: MCB 110L Lecture 3 1/26/2011 Qing Zhong 306 Barker Hall An open reading frame starts with an atg (Met) in most species and ends with a stop codon (taa, tag or tga). Open reading frame mRNA---cDNA Defne Full-length cDNA Peptide hits-protein sequence cDNA sequence Search all the cDNA database Hypothetic ¡ull-length Open reading ¡rame-start Human/mouse genome-Longest reading ¡rame-stop codon be¡ore start codon Defne Full-length cDNA Peptide hits-protein sequence cDNA sequence Search all the cDNA database Hypothetic ¡ull-length Open reading ¡rame-start Human/mouse genome-Longest reading ¡rame-stop codon be¡ore start codon E.coli Expression construct - PET24b Vector Type: Bacterial Stable/Transient: Transient Constitutive/Inducible: Constitutive Expression Level: High Backbone size: 3509 Sequencing Primer: T7 Tag: T7 (Nterm), His (Cterm) Mammalian Selection: Kanamycin Comments:Same as pET21abcd(+) but kanR; a,b,c,d vary by MCS; Fusion protein-delete the stop codon Cin8 Promoter 3’ untranslated region Start codon Stop codon Cin8 Promoter 3’ untranslated region Start codon Stop codon Start codon Stop codon GFP GFP Kinesin subfamily that contains Cin8p Antibody made in rabbits that binds heavy chain of human neuronal kinesin Closely related, not identical...
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This note was uploaded on 09/12/2011 for the course MCB 120L taught by Professor Fairclough during the Spring '08 term at UC Davis.

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Lecture3 - MCB 110L Lecture 3 1/26/2011 Qing Zhong 306...

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