Lecture4 - MCB 110L Spring 2011 Lecture 4 1/28/2011 Prof....

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MCB 110L Spring 2011 Lecture 4 1/28/2011 Prof. Qing Zhong
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Protein Structure
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A specifc protein In the early 1940s Choh Hao Li purified bovine growth hormone. This, by all standards, was the most active and purified growth hormone preparation yet to be obtained anywhere in the world. He also synthesized the first human growth hormone in the world. First commercial GH is called somatotrophin when referring to growth hormone produced by recombinant DNA technology .
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Potential effects
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• Protein Assays: React with side-chains and peptide bond Specifc For protein, but general For type oF protein QuantiFy amount • Enzyme assays Rely on Function oF protein Specifc For type oF protein QuantiFy activity Assays 2HN R 1 O H N O R 2
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Protein determination Beer's law states that the absorbance is directly proportional to the concentration of a solution like beer . Beer’s law Absorbance (A) - concentration (C)
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Beer’s Law I 0 I A=C ε l Where: A = Absorbance C = Concentration ε = extinction coefFcient (solute/protein speciFc) l = path-length hv l The extinction coefficient for a particular substance is a measure of how well it scatters and absorbs electromagnetic radiation (EM waves). If the EM wave can pass through very easily, the material has a
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Lecture4 - MCB 110L Spring 2011 Lecture 4 1/28/2011 Prof....

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