Lecture5 - MCB 110L Spring 2011 Lecture 5 2/2/2011 Qing...

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Unformatted text preview: MCB 110L Spring 2011 Lecture 5 2/2/2011 Qing Zhong 306 Barker Hall Assays, assays, assays a biochemists jeweled tools!!! How much total protein is present? How much of this protein is the kinesin Cin8p? Desired features of any assay for ANYTHING: Simple Quick Accurate Reproducible Sensitive Specific Enzyme assay I-Continuous assays Spectrophotometric- also called colorimetric assays if color is changed. Fluorometric-Fluoescence is when a molecule emits light of one wavelength after absorbing light of a different wavelength. Calorimetric-heat absorbance Chemiluminescent-emission of light by a chemical reaction. ECL, luciferase. Enzyme assay I-Discontinuous assays Radiometric Radiometric assays measure the incorporation of radioactivity into substrates or its release from substrates. Chromatographic Measures product formation by separating the reaction mixture into its components by chromatography Factors to control in assays Salt concentration High salt kills (1-500mM) Effect of temperature...
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This note was uploaded on 09/12/2011 for the course MCB 120L taught by Professor Fairclough during the Spring '08 term at UC Davis.

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Lecture5 - MCB 110L Spring 2011 Lecture 5 2/2/2011 Qing...

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