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Unformatted text preview: Notes for lecture 6 ­8 GSI: Nikki Beginning and End of Replication: 1st step of replication is origin recognition Bacteria—1 OriC sequence in the entire genome, two replication forks to finish copying the circular plasmid Eukaryotes—many origin sequences, linear chromosome OriC sequence structure: AT ­rich region—9bp—9bp—9bp—9bp ATP ­binding allows DnaA to bind the 9bp region, and because there are repeats, multiple DnaA molecules bind (cooperatively) and form a filament  ­ ­> distort the B form duplex ­ ­> favors unpairing of bases  ­ ­> resulting in melting open the AT ­rich region near by  ­ ­> opened AT ­rich region forms a replication bubble with two forks on either side. This mechanism of “ATP binding facilitating protein binding and ATP hydrolysis facilitating dissociation” is common: i.e. clamp loader, and DnaC loadi...
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