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Will this be successful if the dna you want to

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Unformatted text preview: 8. You are studying a wild ­type strain of bacteria that express a restriction endonuclease that recognizes the sequence: a) What type of overhang will this restriction enzyme leave? Blunt, 3’ overhang or 5’ overhang? b) You want to introduce a plasmid from another bacteria into this species. Will this be successful if the DNA you want to introduce is left as is? Why and why not? 9. If a DNA polymerase lacks a 3’ ­5’ exonuclease activity, do you expect it to be more or less accurate than E. coli DNA polymerase I? Explain. 10. Is the 3’ ­5’ exonuclease activity of DNA PolI simply the reverse reaction of the 5’ ­ 3...
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