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PS2 key - MCB 110 Spring 2011 Problem Set 2 1 True or...

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MCB 110 Spring 2011 Problem Set 2 1. True or False _ T _ DNA polymerase can only synthesize DNA in the 5’ 3’ direction. _ F _ Single-stranded RNA is unstable, whereas single-stranded DNA is extremely stable on its own during synthesis. False, ssDNA is not stable on its own during synthesis. It requires SSB (single stranded binding protein) to bind and stabilize it. _ F _ The viral equivalent of DnaA and DnaC is λ 0. False, it’s the viral equivalent of DnaA. __ F _ DNA pol II has 5’ 3’ but not 3’ 5’ exonuclease activity. Only DNA pol I has 5’ 3’ exonuclease activity _ T _ DNA pol III is a multimeric complex, but DNA pol I is monomeric. True, DNA Pol III functions as a dimer that is tethered by a subunit of the clamp loader. DNA Pol I is monomeric. _ T _ Telomerase is a ribonucleoprotein. True, it incorporates a short of piece of ssRNA as an internal template to add on more telomeres 2. Match the term with the definition. There are more terms than definitions. a.) Percentage of shared identity between homologs sequence conservation b.) refers to an experiment performed in a test tube in vitro c.) enzyme that can digest RNA in an RNA/DNA duplex RNase H d.) the potential energy felt by a charge due to the electric field at a point in space electrostatic potential e.) longevity of a covalent structure chemical stability f.) cleaves nucleic acids within the sequence endonuclease Semi Conservative structural stability SSB Exonuclease angstrom DNA helicase Endonuclease DNA supercoil Electrostatic potential In vivo DNA ligase Sequence conservation In vitro DNA gyrase Helicase DNA polI RnaseH Kinetic competence Klenow fragment
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3. Draw a double-stranded DNA with a replication fork and label the following: a. 3’ and 5’ ends b. Direction of fork movement c. Okazaki fragments d. SSBs e. RNA primers f. Lagging strand and leading strand See your notes for diagrams.
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