SectionNotes1a

SectionNotes1a - from Albert Einstein University Monday,...

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Unformatted text preview: from Albert Einstein University Monday, January 24, 2011 Looking into the major groove A H-bond acceptor D H-bond donor M Methyl group M 5’ D A N 4’ N H A CH3 O 2’ 1’ 3’ 2’ Adenine 3’ 1’ Thymine 4’ Dotted line indicates H-bond between Adenine and Thymine. Atoms are colored by element identity. Other than the AT base pair indicated here, bases are represented as simple rings without functional groups shown. Major groove allows access to sequence information that can differentiate AT from TA and GC from CG. More sequence information in the major groove than the minor groove. Monday, January 24, 2011 part of backbone B-DNA part of backbone 2’ up A-DNA (and RNA) part of backbone part of backbone 3’ up 2’ down from Tulane University RNA 2`OH A-form RNA has the 3’ carbon pulled up out of the plane of the Steric Hindrance rest of the sugar ring. In B-DNA the 2’ carbon is pulled up. 3` 5` Won’t fit B-DNA helix Monday, January 24, 2011 phosphodiester bond Steric Clash of 2’OH with backbone causes RNA to adopt C3’ endo sugar pucker which leads to A-form RNA to backbone to backbone to backbone to backbone from Biochemistry, Berg, Tymoczko, Stryer. 5th ed. Ch 27.1 Monday, January 24, 2011 from Lehninger, Principles of Biochemistry, 4th edition Monday, January 24, 2011 from Lehninger, Principles of Biochemistry, 4th edition Monday, January 24, 2011 DNA sequencing Automated DNA sequencing polymerase synthesizes DNA of various sizes but attenuated by fluorescently labeled dideoxynucleotide (in small amounts comparing to dNTPs) from Lehninger, Principles of Biochemistry, 4th edition Monday, January 24, 2011 ...
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This document was uploaded on 09/12/2011.

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SectionNotes1a - from Albert Einstein University Monday,...

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