Key Prac Ex1

Key Prac Ex1 - KEY to PRACTICE EXAM #1 Question 1. Part 1....

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1 KEY to PRACTICE EXAM #1 Question 1. Part 1. Answer: Many actively transcribed genes recruit histone acetyltransferases to their promoters. These enzymes modify histone tails to loosen up the interactions of histones with promoter DNA in nucleosomes, resulting in greater accessibility to the promoter DNA by general transcription factors and RNA Pol II. Therefore, the addition of an inhibitor of histone acetyltransferases to tissue culture cells would expect to cause an inhibition of transcription from many Pol II genes. An experiment using DNA microarray to compare the global gene expression profiles between the inhibitor-treated and untreated cells would be the best experiment. However, one can also use the in vivo transcription assay discussed in class that involves co-transfection into tissue culture cells of a luciferase reporter plasmid and an effector plasmid expressing a transcriptional activator. The luciferase reporter gene is driven by a cellular promoter, which will be activated by the transfected activator (e.g. CREB) through the recruitment of histone
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This document was uploaded on 09/12/2011.

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Key Prac Ex1 - KEY to PRACTICE EXAM #1 Question 1. Part 1....

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