mcb130-fa07-mt1-Unknown-exam

mcb130-fa07-mt1-Unknown-exam - MCB130 Midterm Name SID GSIs...

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MCB130 Midterm Name: September 28, 2007 SID: GSI’s Name: 1 Karsten Weis - Part II [90 points total] Question 1a : Question 1b : Question 2 : Question 3 : Question 4 : Question 5 : Total :
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MCB130 Midterm Name: September 28, 2007 SID: GSI’s Name: 2 1. Peroxisomes are small, membrane-enclosed organelles that function in the degradation of fatty acids and in the degradation of H 2 O 2 . Peroxisomes are not part of the secretory pathway and peroxisomal enzymes are targeted directly from the cytoplasm to the lumen of peroxisomes via a peroxisomal targeting signal (PTS). a. Design a general experimental strategy to identify the PTS in your favorite p eroxisomal p rotein 1 (FPP1). [15 pts]
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MCB130 Midterm Name: September 28, 2007 SID: GSI’s Name: 3
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MCB130 Midterm Name: September 28, 2007 SID: GSI’s Name: 4 1. b. Previous research has shown that many peroxisomal enzymes contain a PTS at their very carboxy-terminus. This signal, termed PTS1, consists of the three amino acids Ser-Lys-Leu. A researcher fuses the ER signal peptide to his favorite p eroxisomal p
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