Methods

Methods - BIO 181 Dr. Sweat Methods The lab experiment for...

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BIO 181 Dr. Sweat Methods The lab experiment for “Biologically Important Molecules” includes the study organic compounds that can be found in most living organisms, such as carbohydrates, proteins, lipids, and nucleic acids. In order to identify these, six separate controlled experiments were conducted, each using positive and negative controls to compare the experimental procedures with the unknown solution. Procedures were followed exactly as detailed in Biology Lab Manual (Vodopich et. al, pg. 57-70). For the first experiment, we performed the Benedict’s test for reducing sugars. We combined seven different 200μL solutions (onion juice, potato juice, sucrose solution, glucose solution, distilled water, reducing-sugar solution, and starch solution) in a test tube with 2000μL of Benedict’s solution and observed the reactions. The second experiment included combining 140μL of iodine with seven test tubes, containing the solutions used in the previous experiment. This was performed to identify starches in the
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