Unformatted text preview: absorbance of four unknown concentrations. The absorbance and concentrations were recorded as follows; unknown Tube #2= 0.826 A / 32.5 mg/mL, unknown Tube #3= 0.432 A / 15.0 mg/mL, unknown tube #4= 0.426 A / 14.0 mg/mL unknown tube #10= 0.754 A / 28.5 mg/mL. For the last procedure, a graph was constructed (see Fig. 3) plotting relative absorption of light versus wavelength that represents the absorption spectrum of Chlorophyll. The proper blank for determining the absorption of Chlorophyll in a plant extract is Acetone. After completing the procedure, we concluded the wavelengths that are least absorbed by Chlorophyll are greens and yellows, while the wavelengths that are most absorbed are blues and reds....
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This note was uploaded on 09/14/2011 for the course BIO 181 taught by Professor Sweat during the Spring '11 term at ASU.
- Spring '11