{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

question5 - TrpR(Trp repressor The gene product of the trpR...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
TrpR (Trp repressor) The gene product of the trpR gene. It contains a helix-turn-helix motif allowing it to bind to DNA. LOV Abbreviation for "light, oxygen, voltage". The LOV domain of a protein photosensor, included in proteins such as the phototropins (light-activated serine/threonine kinases). The LOV domain of A. sativa is known as AsLOV2.[5.3] LOV's typically have a prosthetic FMN (flavin nucleotide) chromophore. Lov-TAP LOV and tryptophan-activated protein. It is the end-to-end fusion product of TrpR and AsLOV2. [5.3] When illuminated by by light, it binds cognate DNA protecting it.[5.2] Presumably, AsLOV2 activates the TrpR and then TrpR would bind to the operator of the trp operon. This allows the fusion product to inhibit trp transcription. The Original Circuit (from 5.1):
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Figure pulled from: A Synthetic Genetic Edge Detection Program Jeffrey J. Tabor, Howard M. Salis, Zachary Booth Simpson, Aaron A. Chevalier, Anselm Levskaya, Edward M. Marcotte, Christopher A. Voigt, Andrew D. Ellington Cell - 26 June 2009 (Vol. 137, Issue 7, pp. 1272-1281)
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}