question7 - The remaining unreplicated fragment of DNA can...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Diagram: The first 160 base pairs at the left end of the genome are identical to the final 160 base pairs at the right end of the bacteriophage’s genome which is the key to solving the linear problem. DNA replication begins at the origin and synthesis of the daughter strand is terminated just before the beginning of the terminal repeat leaving sticky ends. Since DNA polymerase needs an RNA primer to replicate and it can only synthesize DNA in one direction, the polymerase cannot continue past the repeat sequence at the ends of the genomes. The bacteriophage has developed a pathway of annealing multiple replicated genomes together which is possible due to the terminal repeats creating a concatemer. The concatemer can then be cut at specific locations (right of top black and left of bottom black strands) to generate the following individual genome:
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: The remaining unreplicated fragment of DNA can now be completed because it is now in the 5 to 3 direction which is required by DNA polymerase. This regenerates the original DNA of the same length. No DNA is lost. Concatemers of multiple genomes are continually created and individual genomes are cut off and replication is completed over and over again. Diagrams from: Biochemistry 3107 - Fall 2001 Bacteriophage Replication web site The 3' overhangs cannot be replicated. Instead, these are used to fill the gaps to form a long strand. This is broken by an endonuclease that forms 5' overhangs. These can be replicated. Relevant literature (does not need to be cited):
View Full Document

This note was uploaded on 09/15/2011 for the course BIOCH 213 taught by Professor Satterly during the Spring '11 term at University of Texas at Austin.

Page1 / 3

question7 - The remaining unreplicated fragment of DNA can...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online