Forensic_Drug_Testing_2

Forensic_Drug_Testing_2 - Forensic Drug Testing Part 2:...

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Forensic Drug Testing Part 2: GC/MS Confirmation Roger L. Bertholf, Ph.D. Associate Professor of Pathology Chief of Clinical Chemistry & Toxicology
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Screening vs. Confirmation Low cost Fast Semi-quantitative High sensitivity Low specificity High cost Slow Quantitative High sensitivity High specificity
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A confirmatory method should . . . Utilize the most accurate (specific) testing method available Have sensitivity equal to or better than the screening method Be economically feasible Be simple enough to standardize across many laboratories Produce results that are legally defensible
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Chromatography Separation of components based on their. . . Solubility in mobile and stationary phases Terminology: Gas/liquid Liquid/liquid Ion exchange Partition
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Chromatographic separations Stationary Phase Mobile Phase
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Chromatographic separations A B Soluble in stationary phase Long retention time Soluble in mobile phase Short retention time
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Chromatographic separations Detector signal Time B A The resolution of a chromatographic separation is defined as: t/mean peak width
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Chromatographic resolution w (A) w (B) V r 2 ) ( ) ( ) ( ) ( B w A w A V B V R r r s + - =
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Chromatographic resolution • The resolution, R s , is a unitless quantity since it is the ratio of two measures of retention (time, volume, or distance). In general, in order to satisfactorily separate
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Forensic_Drug_Testing_2 - Forensic Drug Testing Part 2:...

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