HAMA_ACS_SLC - Susceptibility of immunoassays to...

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Unformatted text preview: Susceptibility of immunoassays to interference from human anti- animal antibodies: How common a problem ? Roger L. Bertholf, Ph.D. Associate Professor of Pathology University of Florida Health Science Center/Jax Human anti-animal antibodies First described in 1971 by Ammann and Hong Potential to cause positive or negative interferences Anti-isotype or anti-idiotype Anti-mouse antibodies are most common Incidence is not certain Two-site sandwich enzyme immunoassay E E E E E Specimen 2nd antibody E Substrate S P HAMA bridging interference E E E E 2nd antibody E Substrate S P HAMA blocking interference E E E E Competitive immunoassay Specimen Labeled antigen Wash HAMA blocking interference Case Report The laboratory director was consulted by a gynecologist about discrepant CA-125 results on a patient being monitored for recurrence of a surgically removed ovarian tumor. CA-125 results from a referral laboratory had been consistently high and increasing, while results from the hospital laboratory were within normal limits. CA-125...
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HAMA_ACS_SLC - Susceptibility of immunoassays to...

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