Burden, Sierra, Friday G, Genetics, PreLab 1

Burden, Sierra, Friday G, Genetics, PreLab 1 - Bio44X...

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Bio44X Genetics Prelab 1 Name Sierra Burden Day and Section Friday G CA and Intern Lillian and John Title and Objective: 5 points Write out the title and objective for our Week 1 Experiment (Scutoid experiment) below. Title: Using recombination frequency and meiotic mapping to determine the location of the Scutoid Mutation on Drosophila's chromosome II Objective: The purpose of the experiment is to determine the location of the scutoid mutation relative to three other mutations, al , dp , and c . All of the mutations are located on chromosome II of the Drosophila and the exact locations of the al, dp , and c mutations are known. The relative location of the scutoid mutation can be determined by calculating the recombinant frequencies and meiotic mapping. In the experiment, a cross between Sco / Cyo and al, dp , c / Cyo will be conducted. The progeny of the cross will yield Sco / al, dp, c , which is the strain of interest. The other phenotypes will be easily discernible and eliminated because a balancer chromosome will be used. Female flies of the F1 genera- tion will be crossed with al , dp , c / Cyo to observe the recombination that occurs. The fre- quency of the recombination phenotype non-curly, scutoid flies will be used to determine
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This note was uploaded on 09/19/2011 for the course BIOLOGY 44Y taught by Professor Smith during the Winter '11 term at Stanford.

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Burden, Sierra, Friday G, Genetics, PreLab 1 - Bio44X...

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