Burden, Sierra, Friday G, Genetics, PreLab 2

Burden, Sierra, Friday G, Genetics, PreLab 2 - Bio44X...

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Bio44X Genetics Prelab 2 Name: Sierra Burden Day and Section: Friday G CA and Intern: Lillian and John Title and Objective: 5 points Write out the title and objective for our Week 2 Experiment (Gal4-UAS experiment) below. Title: Determining the sufficiency of the eyeless gene in Drosophila melanogaster by etopic expression using the Gal4-UAS system Objective: The purpose of the experiment is to conduct a sufficiency test for the eyeless gene in Drosophila. The gene will be expressed in parts of the Drosophila where it is not usually ex- pressed. This can be accomplished by using the Gal4-UAS system, which allows for target gene expression in a number of tissue or cell patterns. If the eyeless gene is sufficient, then it will be expressed in the areas where it is not usually. In this experiment, the etopic expression will be in the antennae, wings, and legs. If the gene is not sufficient, then the Drosophila will not express eyes in the antennae, wings, and legs. Questions: 15 points
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Burden, Sierra, Friday G, Genetics, PreLab 2 - Bio44X...

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