BS111LPosterIntro - BS111L Int roduction Chlamydomonas is...

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BS111L Introduction Chlamydomonas is often used in biological experiments to observe various cellular activities. Lefebvre et al. (1999) examine why Chlamydomonas is often used because it is eukaryotic, like cells in the human body, but also unicellular with chloroplasts and flagella that make it ideal for studying cellular signaling and responses. Through previous studies and through our own experiments, we know that acid shocking live chlamy causes deflagellation of the cells. Evans et al. (1997) used similar methods for acid shocking chlamy to induce flagellar excision. Neomycin is a known inhibitor that prevents the activity of phospholipase C in the signal transduction pathway, and calcium ionophore is a known activator for deflagellation after acid shocking. Finst et al. (1998) discusses the importance of Ca 2+ in activating the severing of microtubules in flagella, explaining why we use calcium ionophore to activate deflagellation. Also, the results from Quarmby et al. (1992) supported
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BS111LPosterIntro - BS111L Int roduction Chlamydomonas is...

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