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BI+107+Chapter+16 - Chargaff’s Rule was very important to...

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Chapter 16 DNA Structure and Replication
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Griffith Experiment Used mice and bacteria called  Streptococcus  pneumoniae S. pneumoniae  that are pathogenic are smooth  looking (S) S. pneumoniae  that are NOT pathogenic are  rough looking (R). His goal was to show that genetic material was  transferred between the bacteria. Avery  et al.  carried on but was NOT able to “prove” that the  material was DNA. The skeptics assumed DNA to  be too simple to be responsible for all the variation  see in life.
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Hershey-Chase Experiment Used bacteriophage T2, E. coli and  radioactive sulfer ( 35 S) and radioactive  phosphorus ( 32 P) Did “prove” that DNA was the genetic  material
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The structure of DNA Watson, Crick, Wilkins and Franklin were  all involved in determining the structure of  DNA.
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Unformatted text preview: Chargaff’s Rule was very important to this discovery. Chargaff found that the amount of adenine always equals thymine and the amount of cytosine always equals guanine in DNA. The amounts change in different species but A=T and C=G. This explains why Chargaff’s Rule is true DNA Structure How does DNA replicate? • Watson and Crick proposed that one part of the DNA can act as a template for a new strand. • Other models for DNA replication were also proposed. • To fully answer this question Meselson and Stahl did an experiment to answer which of the models is correct. Enzymes or proteins involved in DNA replication Don’t worry about DNA pol I Enzymes/ proteins you need to know Overview of DNA replication Fixing Thymine dimers made by UV light...
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