lecture7 - Detergents amphipathic/amphiphillic molecules...

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Detergents – amphipathic/amphiphillic molecules that have a limited solubility and above a critical concentration form micelles (CMC). micelle monomer When micelles are present, there is always free monomer present too If detergent concentration is less than CMC, monomers are in solution If detergent concentration is above CMC, …..
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Detergents are typically broken down into 4 classes:
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detergent CMC (mmol) Aggregation # SDS 2.3 84 OG 25 27 CHAPS 8 11 LysoPC .007 186 Tween 80 Tween 80 0.012 58
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* *
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General Properties of Classes of Detergents for Membrane Protein Studies *SDS will help stabilize an all a-helix peptide/protein.
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Detergent Elimination Strategies: Dialysis Dilution Size Exclusion Biobeads (hydrophobic adsorption) Membrane Protein Reconstitution Solubilizing Membranes Protein Purification Schemes
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Features of the 4 stages:... Ratio of detergent/lipid important, [] detergent important
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Turbidity and Light Scattering: An increase in turbidity/light scattering is an indication of a larger
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